WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction

This definitive and essential source of reference has been thoroughly updated and revised to meet the requirements of all laboratories involved in the analysis of human semen. The book sets out the fundamental laboratory techniques that should be employed in the diagnosis of male infertility. The text includes descriptions of how to construct a conventional semen profile and provides standardized protocols for performing several optional diagnostic procedures. Previous editions of this volume have established themselves as the gold standard in the area of fertility investigation and treatment and this new edition will set new standards for the new millennium.
Contents

Acknowledgements; Abbreviations; 1. Introduction; 2. Collection and examination of human semen; 2A. Standard procedures; 2B. Optional tests; 2C. Research tests; 3. Sperm preparation techniques; 4. Quality control in the andrology laboratory; 5. Sperm-cervical mucus interactions; Appendices. IA. Reference values of semen variables; IB. Nomenclature for some semen variables; II. Safety guidelines for the andrology laboratory; III. Methods for detecting leucocytes; IV. Sperm vitality techniques; V. Papanicolau staining procedures modified for spermatozoa; VI. Shorr staining procedure for sperm morphology; VII. Rapid staining procedure for sperm morphology; VIII. Immunobead test; IX. Mixed antiglobulin reaction test (MAR test); X. Measurement of zinc in seminal plasma; XI. Measurement of fructose in seminal plasma; XII. Measurement of neutral a-glucosidase in seminal plasma; XIII. Sample instructions and record form for semen analysis; XIV. Computer-aided sperm analysis (CASA); XV. Protocols for the zona-free hamster test; XVI. Measurement of reactive oxygen species generated by sperm suspensions; XVII. Induced acrosome reaction assay; XVIII. Sperm preparation techniques; XIX. Quality control (QC) procedures; XX. Sample record form for cervical mucus scoring and postcoital test; XXI. Sperm-cervical mucus interaction: the capillary tube test; XXII. Basic requirements for an andrology laboratory; Bibliography; Index.
Reviews

"This manual would be a valuable companion to both small and large medical laboratories, as well as to andrology labs...This is a good manual and I would personally recommend it to any laboratory that performs semen analysis. It would also be a good reference tool in forensic and artificial insemination laboratories." Canadian Journal of Medical Laboratory Science

"The WHO Manual should have a prominent place on the benches of all laboratories dealing with human semen and on the shelves of clinicians dealing with subfertile couples... The new fourth edition or the 'gold book,' as I am sure it will be called, will then continue to be the benchmark for setting more rigorous standards in the future." Human Fertility

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