Molecular Biomethods Handbook

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.

Molecular Biomethods Handbook 2nd Ed



Contents

PART A: NUCLEIC ACID BIOMETHODS

1 The manipulation of nucleic acids: basic tools and techniques – Gayle Corkill and

Ralph Rapley

2 Restriction Enzymes – Gareth J.S. Jenkins

3 Principles and Medical Applications of the Polymerase Chain Reaction – Birmal D.M.

Theophilus

4 Probe Design, Production, and Applications – Marilena Aquino de Muro

5 Southern Blotting as a Diagnostic Method – Bronwen Harvey and Pirkko Soundy



6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega

7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid

analysis - Kim Hung Leung and Shea Ping Yip

8 Denaturing Gradient Gel Electrophoresis (DGGE) – Jeroen H. Roelfsema and Dorien

J.M. Peters

9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and

Shea Ping Yip

10 Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic

characterization of different organisms - Lúcia Maria da Cunha Galvão and Eliane

Lages-Silva

11 Quantification of mRNA using Real Time RT-PCR – the SYBR solution - David

Sugden and Patricia de Winter

12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing – Anu

Suomalainen and Ann-Christine Syvanen

13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila

Kousoulidou and Philippos C. Patsalis

14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley

15 Comparative Genomic Hybridization in Clinical and Medical Research – Peng-Hui

Wang, Yann-Jang Chen and Chi-Hung Lin

16 Subtractive Hybridization – Craig Winstanley

17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire

18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice -

Jonathan D. Smith

19 cDNA Microarrays – Phillip G. Febbo

20 Mapping Techniques – Simon G. Gregory

21 Single Nucleotide Polymorphisms Overview – Nameeta Shah

22 Bioinformatics Tools for Gene and Protein Sequence Analysis– Bernd H.A. Rehm and

Frank Reinecke



PART B: PROTEIN AND CELL METHODS

23. Protein Electrophoresis - David Sheehan and Siobhan O’Sullivan

24. Protein blotting - Patricia Gravel

25. Capillary electrophoresis of proteins - Mark Strege

26. Autoradiography and fluorography - Bronwen Harvey

27. Mass spectrometry of proteins & peptides - Kenneth G. Standing

28. Post-translational modifications - Christoph Kannicht and Birte Fuchs

29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung

30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu

31. Glycoprotein analysis - Terry Butters and David C.A. Neville

32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields

33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar

34. Antibody Phage display - Rob Aitken

35. Protein engineering - Thomas Willemsen, Urs B. Hagemann,

Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Müller and Katja M. Arndt

36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Müller

37. Enzyme Linked Immunosorbent Assay - John Crowther

38. Epitope mapping - Glenn E. Morris

39. Quantum dots - Charles Hotz

40. Ion exchange chromatography - David Sheehan

41. Size exclusion chromatography - Paul Cutler

42. Hydrophobic interaction chromatography - Paul A. O’Farrell

43. Affinity chromatography - Adam Charlton and Michael Zachariou

44. High Performance Liquid Chromatography

(HPLC) of Proteins and Peptides - Tzong-Hsien Lee and Marie-Isabel Aguilar

45. Amino acid analysis - Ian Davidson + Paula O’Connor

46. Surface plasmon resonance - K. Scott Phillips and Quan Cheng

47. Macromolecular Crystallography - Bernhard Rupp and Kathrine A. Kantardjieff

48. Microchip Devices for Bioanalysis - Anna Kinsella and Shelley D. Minteer

49. Mammalian cell culture - Simon Langdon

50. Plant tissue culture - V.M. Loyola Vargas, C. De-la-Peña, R.M. Galaz-Avalos and F. R. Quiroz-Figueroa

51. Stem cells and Regenerative Medicine - Mohan C. Vemuri and Chellu S. Chetty

52. Cryopreservation : Conservation of Bioresources

at Ultra-Low Temperatures - J.G. Day, K. Harding,

J. Nadarajan and E.E. Benson

53. Magnetic resonance imaging (MRI) - Pottumarthi V. Prasad and Pippa Storey

54. Electron microscopy - John Kuo

55. Confocal microscopy - Guy Cox

56. Laser microdissection - Graeme I. Murray

57. Biomedical uses of Flow cytometry - James L. Weaver and Maryalice Stetler-Stevenson

58. Immunomicroscopy - Constance Oliver

59. In-situ hybridization - Ian A. Darby and Tim D. Hewitson

60. High throughput screening - William P. Janzen

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