Manual of Assisted Reproductive Technologies and Clinical Embryology

This manual is a comprehensive guide to Assisted Reproductive Technologies (ART) and Clinical Embryology. Beginning with an introduction to ART, the book discusses embryology and provides a step by step description of different types of ART. With contributions from international experts, this book includes a DVD illustrating different laboratory and cryobiology protocols. A section on the challenges of ART and possible problems that may be encountered is also included.

Section -1
Understanding ART—Embryologist Outlook
1. Development of Gonads and Germ Cells
2. Sperm Preparation Techniques
3. Setting up of an ART Center
4. Classical CO2 Incubator— Heart and Soul of
An IVF Laboratory
5. Media in ART
6. Gametogenesis and Microscopic Structure of
Gametes and Early Embryo
7. Tuberculosis and Laboratory Perspective 8. The Importance of Water Quality in IVF
Laboratories
9. Oxidative Stress and ART
10. ICMR Guidelines
11. Troubleshooting in ART: Laboratory
Perspective
12. OpticsandART
Section-2
Burning Issues in Human Embryology—Revisited
13. Multifetal Pregnancy Reduction
14. Setting up of a Viable Cryobank
15. Blastocyst Culture
16. Introduction to Study of Semen Analysis 17. A Randomized Controlled Study of Human 1. Day 3 Embryo Cryopreservation by Slow
Freezing or Vitrification
18. Comparison of Open and Closed Methods for
Vitrification
19. Safe Cryobanking
20. Cytogenetics of Male Infertility
21. Ultrasound Assessment of Endometrial
Receptivity, Oocyte and Embryo Quality 22. Introduction to Sperm Morphology
23. Preimplantation Genetic Testing—Clinical
Applications
24. Sperm Preparation for IVF and ICSI 25. Anesthesia and Assisted Reproductive
Technologies
26. Male Infertility—Is it Difficult to Conquer? 27. FertilityPreservationinFemaleCancer
Patients—A Review
28. Human Embryonic Stem Cells— Role in
Regenerative Medicine
29. Single Embryo Transfer 30. Spindle View
31. Analysis of Fertilization and
Embryo/Blastocyst Grading
32. Preimplantation Genetic Diagnosis
33. Utilization of High-Security Straws for Embryo
Freezing in an In Vitro Fertilization 34. Cloning and ART
35. EmbryoTransferSimplified
36. EmbryoCulture—NewStrategies
37. Embryo and Blastocyst Culture
38. Embryo Transfer
39. PhysiologyandCultureoftheHuman
Blastocyst
40. SingleBlastocystTransfer: AProspective
Randomized Trial
41. Intracytoplasmic Sperm Injection: Revisited 42. Karyotyping and ART
43. Oocyte and Maternal Inheritance
44. In Vitro Maturation—Current Scenario
45. Y Chromosome and Its Role in Male Infertility 46. Fishandart
47. The Role of Sperm in Normal Embryogenesis 48. TUNEL Assay for the Assessment of Sperm
Chromatin Damage 49. ICSI—An Overview
Section -3
Pelvic and Reproductive Disorders—Embryology Outcome
50. ImmunologyandReproductiveDisorders 51. Polycystic Ovary Syndrome
52. Ovarian Hyperstimulation Syndrome
53. Laparoscopy and Fertility Enhancement 54. Endometrium in ART
55. GenitalTuberculosis
56. Endoscopic Complications in ART
Section 4
Art Step by Step—Beginner’s Guide
57. Culture Media
58. Air in the Laboratory
59. Disposables in ART
60. Ovum Pick-up
61. Insemination and IVF
62. Semen Preparation in IVF
63. Analysis of Fertilization
64. Embryo Culture
65. Intracytoplasmic Sperm Injection
66. SemenBanking
67. EmbryoTransferStepbyStep
68. Brief View of Cryobiology
69. EmbryoSlowFreezing
70. Embryo Thawing
71. Oocyte Vitrification
72. OocyteWarming
73. OocyteSlowFreezing
74. Protein-free Media
75. OvarianCortexFreezing
76. Fertility Issues in Gynecological Cancers 77. Embryo Vitrification
78. Embryo Reduction: Our Experience
79. Testicular Tissue Preservation
Section - 5
Challenges in Art—A clinician’s Perspective
80. Recurrent Implantation Failure
81. Medical Management of Ectopic Pregnancy 82. HydrosalpinxinART
83. EjaculationanditsDysfunction
Appendices Index